FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_1.00_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_1.00_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7122888
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT201910.2834664815732046TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA29450.058.82716436
TATGCCG30100.055.2359348
ACGTAAT31050.054.89406237
CGTATGC32200.052.06839846
GTATGCC32900.050.85452747
TCGTATG34150.049.196745
CGTAATC31150.048.98831638
CCGTACG37250.047.35294333
CGTACGT37000.046.82225434
CTCGTAT32950.046.3144544
CACGTCT39500.044.92174514
ACCGTAC40100.044.07473832
ACACGTC40650.043.9091913
ACGTCTG41450.042.89285715
AGTCACC43150.042.0137728
ATGCCGT39450.041.61217549
TAATCTC38150.039.17450740
CAGTCAC46850.038.3222127
GTACGTA46650.037.66231535
GTCTGAA47750.037.45331217
Produced by FastQC (version 0.11.5)