FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_0.50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_0.50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9553760
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT123290.12904866774966087TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA30100.041.74259636
TCCGTAT31350.039.40880237
TATGCCG27750.038.72228648
CGTATGC27450.038.63544546
CGTATCT28150.038.0459339
CCGTATC29900.037.57460438
TATCTCG29300.036.07503541
CACGTCT37750.035.3235314
GTTCCGT35650.035.04775635
AGTTCCG37650.033.2787734
CACACGT40450.032.70648612
GTATGCC32800.032.6537647
ACACGTC42250.031.39574613
CGTCTGA42900.031.08289516
ACGTCTG42800.031.0739115
TCGTATG34250.030.96410845
AGTCACA42050.030.37918728
CTCGTAT34650.029.79856344
CAGTCAC45550.028.6595727
TCACAGT47300.027.15527330
Produced by FastQC (version 0.11.5)