FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_0.50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_0.50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500082
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT101350.11923414385884748TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21650.042.19462648
CGTATGC21800.042.064646
CACAGTC25400.037.0657431
GTATGCC24800.036.69388247
CACGTCT27650.034.17672314
TCGTATG27500.033.2182945
ACACGTC28800.032.81221813
ACGTCTG30350.030.7903315
CTCGTAT28000.030.37509744
AGTCACA31450.030.15801428
ACAGTCA31350.030.14257232
CAGTCAC36650.027.5980227
CACACGT34300.027.55078312
ATGCCGT34150.027.15999649
GTCACAG36350.025.6112829
CGTCTGA38250.025.43724316
GTCTGAA40000.023.71173917
TCACAGT39800.023.47914530
GCCGTCT39600.023.4220751
TCGGAAG42200.022.5618343
Produced by FastQC (version 0.11.5)