FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_0.25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_0.25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9525235
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22500.037.4892244
TATGCCG22500.037.3346446
GTATGCC25900.032.97326345
TCGTATG26800.031.86528243
CACGTCT29850.030.83682314
ACACGTC32900.028.1908513
ACGTCTG32450.028.15021315
CTCGTAT25950.028.0534642
AGTCACT32450.027.718528
ACTGACC35400.025.21102532
CACACGT37850.024.13419312
ATGCCGT36850.023.36593447
CAGTCAC42450.022.83787527
CGTCTGA40400.022.26422916
CACTGAC40400.021.83095731
TCGGAAG45200.019.9029163
GTCACTG45050.019.73297329
GCCGTCT43950.019.67087249
GTCTGAA47300.019.60822917
TCTGAAC49000.018.64223718
Produced by FastQC (version 0.11.5)