FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1.250_0.25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1.250_0.25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7952770
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG17500.044.19881432
TATGCCG17800.038.54095546
CACTTAG19950.038.42001731
CGTATGC18500.036.70310244
TCACTTA21100.036.32603530
GTATGCC21400.032.54728745
CACGTCT25400.031.96704114
ACACGTC25800.031.74313413
ACGTCTG26000.030.96012315
TCGTATG21700.029.5164543
TTAGGCA26700.029.10052534
CTCGTAT21150.027.96703142
AGTCACT28300.027.70191628
CAGTCAC35550.025.10433827
ATGCCGT28000.024.50103647
CACACGT33600.024.16586312
CGTCTGA36450.022.18005416
GTCACTT35850.021.86831329
GTCTGAA37450.021.8681617
CTTAGGC37350.020.8963633
Produced by FastQC (version 0.11.5)