FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_2.00_R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_2.00_R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14922639
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC209810.14059845580932434TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC43050.044.5537244
TATGCCG43350.044.32687446
TCGTATG49050.039.38862643
GTATGCC49650.038.6311745
CACGTCT53400.037.8826114
ACACGTC55550.036.03849813
ACGTCTG58600.034.4612915
CTCGTAT48600.033.9198542
AGTCACC61150.033.0238928
CAGTCAC65000.031.33708227
ATGCCGT62850.030.68538947
CACACGT66250.030.323712
CTAATCT56750.029.17110837
GTCTGAA73000.027.71129417
GCCGTCT73950.026.22189949
ACTAATC63950.025.83197836
AGCACAC81050.025.5637710
CAACTAA80550.025.07036434
ATCGGAA81100.024.9909232
TCGGAAG81550.024.8526883
Produced by FastQC (version 0.11.5)