FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_2.00_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_2.00_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11190395
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC314620.2811518270802773TruSeq Adapter, Index 7 (97% over 35bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT181600.1622820284717385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41350.058.06741746
ACGATAT43350.058.04923635
GTATGCC41900.057.47149745
TCGTATG42000.057.00055343
CTCGTAT40500.056.69165442
CACGTCT47900.056.0416314
ACACGTC48250.055.41750313
CGATATC43650.054.92411436
ACGTCTG49550.054.0339515
CGTATGC45850.052.97832544
ATGCCGT45700.052.6933947
GCCGTCT47800.050.0125149
CTGAACT54500.049.51158519
CCACGAT53050.048.0940233
ACCACGA53250.047.84766432
TGCCGTC50950.047.26395848
CACGATA54100.047.16101534
GAGCACA59250.045.483859
GAACTCC59000.045.3200421
TATCTCG51600.045.1740539
Produced by FastQC (version 0.11.5)