FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_2.00_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_2.00_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13087240
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC460110.3515714543326171TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71450.057.16792746
CGTATGC73500.055.1440244
GTATGCC77150.053.30637445
TCGTATG77700.051.7572543
CACGTCT87350.050.36470814
CTCGTAT70500.050.19120442
ACTCAAT86400.049.37935
ACACGTC90800.048.64398613
ATGCCGT85450.047.92450347
AGTCACC92350.047.4099428
ACGTCTG92950.047.2550615
CCACTCA92800.047.06707833
CAGTCAC95150.046.0515827
CTCAATC82700.044.7748536
GTCTGAA101500.043.4122217
ACCACTC101500.042.96379532
CACTCAA102150.042.519134
GCCGTCT93450.042.0989149
CACACGT105850.041.89299412
TGCCGTC96750.041.45887448
Produced by FastQC (version 0.11.5)