FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_1.00_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_1.00_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8630563
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG17400.043.04393433
AGTCACG18400.041.08500728
ACGTTTC20000.037.79820632
CACGTTT22550.033.6791131
TTCGGAA23200.033.0376136
TTTCGGA23150.032.80640835
GTCACGT25450.029.56637829
CACGTCT28300.027.57867814
ACACGTC30950.025.8958313
TCGGAAT29600.025.65786437
CACACGT30750.025.60898612
CAGTCAC33250.023.89374227
ACGTCTG33150.023.75480515
CGTCTGA33350.023.40245816
GTTTCGG33000.023.1202534
TCGGAAG34750.022.6646043
GTCTGAA37350.021.36457317
ATCGGAA37350.020.9935262
TCACGTT36550.020.7787730
TATGCCG12150.019.01364748
Produced by FastQC (version 0.11.5)