FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_1.00_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_1.00_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9520526
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT136690.14357400000798273TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG26050.047.56082528
TATGCCG27050.042.82939548
CGTATGC27300.042.4369646
TCACGTG34050.036.79781330
GCCTTAT33350.036.3114237
GTCACGT34550.036.06268329
GTATGCC32250.035.8148547
TATCTCG33150.033.99729541
TCGTATG34000.033.25048445
CACGTCT42800.031.56455214
ACACGTC42500.031.37577213
ACGTCTG43700.031.07466115
CTCGTAT34350.030.97570444
CACGTGG41800.030.22644431
CCTTATC38200.030.14394238
CACACGT44800.029.92136812
CAGTCAC44100.029.04666927
ACGTGGC44900.027.74979632
ATGCCGT42050.027.30165949
GGCCTTA46400.026.85285436
Produced by FastQC (version 0.11.5)