FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_0.50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_0.50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113369
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC117240.1159257612374274TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24900.044.98201846
CGTATGC27200.042.2063944
GTATGCC29000.039.5866845
TCGTATG29650.038.1286243
CACGTCT32750.037.29594814
ACACGTC34300.035.71277213
CTCGTAT28000.034.62524442
ACGTCTG36000.034.12338315
TGTAATC30650.033.1147536
AGTCACC41100.030.82579228
CTTGTAA39050.030.74209434
ATGCCGT37300.030.1220547
TTGTAAT39900.029.9117535
CAGTCAC44400.028.4558627
TAATCTC37100.027.35827838
GTCTGAA45450.026.95128817
GCCGTCT43000.025.72238349
CACACGT48900.025.19345712
CTGAACT48900.025.12138719
CCTTGTA50400.024.1661233
Produced by FastQC (version 0.11.5)