FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_0.50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_0.50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9678789
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19450.029.87306246
CACGTCT35200.029.23164714
CGTATGC18800.029.04350944
TATCTCG21100.028.69703339
ACACGTC37850.027.00011413
CACACGT38450.026.94287912
ACGTCTG38650.026.89401215
AGTCACT36650.026.6427428
GTCACTA37350.026.14354529
TCACTAG37500.026.132330
CGTCTGA39550.025.75106216
CACTAGC38850.024.6837131
CAGTCAC40850.024.24616427
TAGCTTA41450.023.21995234
TCGGAAG44250.023.0193713
GTATGCC25550.022.46660645
GTCTGAA48050.021.70558517
ATCGGAA47600.021.693572
GCACACG51100.020.75249911
CTCGTAT27100.020.14795142
Produced by FastQC (version 0.11.5)