FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_0.25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_0.25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9901336
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20750.037.78448546
GTATGCC22400.034.68804645
CACGTCT29100.034.39742714
CGTATGC22400.034.3755444
TATCTCG23900.032.217139
ACACGTC31500.031.66556413
ACGTCTG32050.031.44976215
TCGTATG24300.031.3995143
CTCGTAT25450.030.53031242
CGATGTA34850.028.7223934
AGTCACC39100.027.39007428
TGTATCT31750.026.12533837
CAGTCAC42000.025.66551427
CACCGAT38550.025.51143331
CACACGT41450.024.82447612
CCGATGT40500.024.36952233
ATGCCGT34050.022.30634747
GTCTGAA45850.022.28907417
GTATCTC35600.022.21855238
CGTCTGA46950.021.46879216
Produced by FastQC (version 0.11.5)