FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0.625_0.25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0.625_0.25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11984429
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC184630.15405823673368169TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC34350.041.57373444
TATGCCG35250.040.90997346
GTATGCC38700.036.9910145
TCGTATG40750.035.21585543
CACGTCT46000.032.79207214
CTCGTAT37850.032.27262542
AGTCACA47800.031.26395828
ACACGTC48650.031.0780613
ACGTCTG48650.030.93392415
GTCACAT46850.030.85207729
ATGCCGT51050.028.31676147
ATCACGA59250.026.52195734
CAGTCAC59800.026.5120227
CACACGT58300.025.99406212
GCCGTCT55950.025.96205949
CGTCTGA60650.024.87094116
GTCTGAA64300.024.05786517
CACGATC49950.023.96333536
TGCCGTC61100.023.77376648
TCACGAT65450.023.36827335
Produced by FastQC (version 0.11.5)