FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956363
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG1600.001258749917.66755
GGGGAAT2709.66084E-615.8030851
CCGGGAT4007.921699E-915.4080091
CGGGGGG1900.0038038514.9713441
GGGGATC3002.6720732E-514.1911332
CGGGAAT4856.7557266E-913.6851471
CTCTAGT2802.3826603E-413.5043566
GGGGCAC3054.7825347E-412.4352151
GGGCACT3156.392835E-412.0136582
TCTAGTT3258.378465E-411.6327457
CCGGGAG3000.005962014711.062161
CACTAGG1750.001282990711.05563994-95
GGCTTAT3000.006066256711.0364243
CACGACT3500.001516076710.8364011
CGGGGAT4407.728127E-510.7748311
CATTCTA5253.8265716E-610.7685814
GCTTATA3550.001797971410.616914
GGATCCA4004.0693447E-410.6003214
GGGAATC3700.002452386810.2278432
TCTAGAC4206.2009826E-410.126727
Produced by FastQC (version 0.11.5)