FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_FlyWT0718T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_FlyWT0718T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15944853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC355410.2228995149720101TruSeq Adapter, Index 3 (100% over 50bp)
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG174740.10959022325260698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA104850.018.392368
GTACTAA109000.017.3870629
TATGCCG128000.015.54833646-47
TTCGTAC126750.015.3296716
CCTTTCG127050.014.9950393
CGTATGC134000.014.95848244-45
CGCGGGG23000.014.0478341
CTTTCGT138050.014.0408094
CTTGAAA143900.013.99538660-61
CGGGGAT50300.013.6970911
CTCGTAT147050.013.69558742-43
GCCGTCT136650.013.50396248-49
GTATGCC137950.013.49721544-45
TCGTATG138850.013.32420842-43
TCCTTTC147900.013.2346482
TTTCGTA151150.013.2007495
GGGGTAT31600.013.0815891
GGGGTCT30600.012.7327061
ACCTAGC15050.012.6264416
GTCCTTT157300.012.5960861
Produced by FastQC (version 0.11.5)