FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_FlyWT0629T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_FlyWT0629T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18801876
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA110300.018.68948
GTACTAA112050.018.3551189
TTCGTAC130650.016.2538646
CCTTTCG136700.015.4656613
CTTTCGT145600.014.324184
CGCGGGG31100.014.2087971
TCCTTTC157850.013.7849392
GTCCTTT161400.013.2772391
TTTCGTA165900.013.2298255
ACCTAGC16350.012.7847846
GACCTAG17050.012.2598955
CGGGGAT66750.011.4606531
GGGGTAT32650.011.35131551
GGGGTCT39500.011.187181
ACTCTAG31400.010.7420515
CGGGTCT61250.010.3952151
TCGTACT222300.010.3202167
CGCAAAT152150.010.2744521
ACCTTAG33100.010.1903456
GGGGGAT55750.010.1423091
Produced by FastQC (version 0.11.5)