FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_FlyOrco0718T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_FlyOrco0718T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083645
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG164180.11657493496889478No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT141170.1002368349954859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA97800.018.0184258
GTACTAA99750.017.6185689
TTCGTAC112800.016.0039526
GGGGTCT27300.015.838471
CCTTTCG117900.015.0304653
CGGGGAT49250.014.4716981
CTTTCGT121800.014.4317664
GGGTCTT106500.013.9630842
TTTCGTA134150.013.6693965
TCCTTTC134800.013.2520972
GTCCTTT136100.012.7429281
CGCGGGG22900.012.4494511
GCCGGTT45050.011.9184041
CGGGTCT43550.011.8924891
CTCGTCT121850.011.7723519
TACACCC137850.011.6827665
GCTACAC138650.011.5474853
CGGGGGT22650.011.5379581
CGGCTAC149700.011.3948111
GCGGGTT32650.011.3513071
Produced by FastQC (version 0.11.5)