FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntWT0629T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntWT0629T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19825385
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA311470.15710665896273893No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC244810.12348310007598844TruSeq Adapter, Index 2 (100% over 50bp)
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT239410.12075931942809685No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT239030.12056764597509707No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT223860.11291583996981647No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT208310.1050723605115361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG57800.027.6991885
GGTCATA105950.023.9897964
AGTGACC58950.022.3234316
CTCTAGT74800.021.4674286
GAGGTCA104850.021.1158052
CTAGTTG76800.020.8424728
TGACCGC68300.019.9591398
CACCTTA94200.019.4671025
TAATCCC130550.019.355999
AGGTCAT117750.019.0037923
TCTAGTT96650.018.2819147
CAGTGAC75900.017.6511215
CCTTAGA105700.016.9862467
CGAGGTC133750.016.5906581
GTACTCT99550.016.3694133
CTTAGAG111150.016.2388298
GTCATAA161300.015.9046565
GTGACCG88600.015.3324987
TGTACTC108650.015.26112
GGAACAG104500.015.0505461
Produced by FastQC (version 0.11.5)