FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113395
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA53850.019.6737044
TAATCCC72400.014.236469
GTCATAA78050.013.3300585
AGGTCAT65200.012.8245723
GGGGAAT31950.011.4513761
GAGGTCA80050.010.8612512
CGGGGGG26350.010.6392051
CGGGAAT65850.010.174221
CGGGTAT43950.010.0545331
CTCGGGT60850.09.9170141
ATAATCC105900.09.9123628
GGGGGAT33150.09.4601541
GGGAATC48450.09.3157942
GACCCTA32200.09.2932057
TCTAGAC71100.09.0187227
CGGGCAT37100.08.96524051
GGGGTCA30050.08.853882
CCCAATT77650.08.8116831
CCGGAAT52350.08.804261
CCCGTAT27150.08.7506031
Produced by FastQC (version 0.11.5)