FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15269513
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA58900.016.9384884
TAATCCC75750.013.6695719
AGGTCAT69900.011.6225383
GTCATAA88400.011.446945
CGGGGCT26250.011.4033671
GGGGAAT44200.011.1797711
GGGGCTA9205.456968E-1210.8448832
CCCGGAT43750.010.1001251
CGGGTAT44700.09.6728781
TCTAGAC68950.09.2999497
ATAATCC115550.09.0434428
CCCTAAG64400.08.88755354-55
CGTACAA150050.08.8017885
GGGAAAT95350.08.7702871
CGGGGGG26700.08.7198031
CTAGACT69900.08.6978888
GAGGTCA96300.08.68322
CGGGGAT73300.08.6212361
CGGGAAT70100.08.6081061
CCGGAAT61200.08.5401031
Produced by FastQC (version 0.11.5)