FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29534172
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA111950.017.5265254
TAATCCC143300.013.6562649
AGGTCAT125600.012.9743213
GTCATAA161950.012.1151685
GAGGTCA170300.011.0476772
CGGGAAT129450.010.3877071
CCGGGAG178800.010.1780971
ATAATCC206600.09.8859648
CTAGACT148150.09.8748378
TCTAGAC150150.09.8382067
GACCCTA68750.09.4651777
CGGGGCT47700.09.3636411
GGGGAAT69700.09.2713171
CGGGGGG53450.09.1564021
GGGAATC99000.09.0702052
ATTCTAG193450.08.9145255
CCGGGAT152500.08.9111041
CGGCTCT141250.08.7798551
GTTTAAT288450.08.7634761
CCCAATT149700.08.6651521
Produced by FastQC (version 0.11.5)