FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18815750
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA88650.019.9357814
TAATCCC109800.015.7030669
AGGTCAT96950.015.142283
GTCATAA123200.014.2289985
GAGGTCA113650.012.9593852
CGGGGAT61800.012.0710451
ATAATCC156700.011.6094058
GGGGAAT52700.010.6391061
TCTAGAC102700.09.7587067
CGAGGTC155000.09.5643751
TCATAAT192250.09.4643566
CTAGACT103600.09.3988418
CCGGGAT77600.09.3683571
CGGGAAT94950.09.3078991
GTTTAAT217650.09.2782051
CCCTAAG108900.09.268537554-55
GGGGATC48350.09.2368422
CGGGGAG69000.08.9521631
AATCCCA128050.08.75412210-11
GACCCTA55400.08.7452177
Produced by FastQC (version 0.11.5)