FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18038334
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA62350.016.1537694
TAATCCC80850.012.631079
GTCATAA89900.011.3088615
AGGTCAT74750.011.0591293
CGGGGGG35950.010.0450131
CGGGAAT84450.09.7337971
CCGGGAG98050.09.4982661
CGGGCAT50800.09.4469911
GGGGGAT40800.09.3167761
GGGGAAT47900.09.2253591
GAGGTCA112250.09.0577172
GGGGATC51400.08.873592
CGGGGTT33050.08.7698911
TCTAGAC80650.08.6575437
CGGGGCT30650.08.5264461
ATAATCC123150.08.5239258
GGGGCAT28700.08.4435311
GGGAATC63300.08.2562022
CTAGACT82900.08.2506798
CGGGTAT55000.08.1208411
Produced by FastQC (version 0.11.5)