FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18257229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA70550.018.5187574
TAATCCC92550.013.7030059
GTCATAA102250.013.0094525
AGGTCAT86700.012.3296023
GGGGAAT55350.010.9022111
GAGGTCA99850.010.8488362
CGGGGAT65800.010.6149991
GGGGGAT44950.09.8306391
GGGGATC52800.09.6282252
ATAATCC142450.09.3029948
TCTAGAC86300.09.1915057
CGGGGAG68950.09.1652821
CCCTAAG81500.08.80035854-55
CGGGCAT56200.08.6236731
CCGGGGT32300.08.5320611
CTAGACT86500.08.5113138
CGGGAAT92600.08.4664521
CGGGGGT34850.08.4531271
TCATAAT165500.08.439446
CCCAATT98650.08.2843771
Produced by FastQC (version 0.11.5)