FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15538822
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA63100.017.467564
TAATCCC86600.012.6151179
AGGTCAT72900.012.1218863
GTCATAA96200.011.7534975
GAGGTCA81250.010.8179342
GGGGAAT41450.010.3171241
CGGGAAT77900.010.0643841
CTAGACT81600.09.7791368
ATAATCC121200.09.3664858
TCTAGAC84500.09.3310987
CGGGGCT26700.09.254071
CCGGGAT64850.09.1588461
GGGGTAT19750.09.1423371
GGGGGAT34600.09.0637641
CCGGAAT61350.08.9842971
GTTTAAT175650.08.6565051
GGGAATC59100.08.6018572
CGGGGAT61950.08.5904791
CGGGGAG58150.08.4164351
GGGCAAT48250.08.370711
Produced by FastQC (version 0.11.5)