FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l02_n01_AntOrT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l02_n01_AntOrT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15689637
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA56800.018.3176254
ACTCTAG44700.014.560565
AGGTCAT66650.012.5457193
TAATCCC78050.012.5364619
GAGGTCA72050.011.8695442
GTCATAA87950.011.6676385
CTAGTTG46250.011.6050598
CGTACAA168950.011.5008565
CCTTAGA59100.011.41251859
CACCTTA59350.011.3644467
CTCTAGT50700.011.3381576
TTCGTAC159600.010.9248693
GGGGAAT45750.09.9707591
CGGGAAT73000.09.893931
GTTTAAT143200.09.8551111
TCGTACA186350.09.6878544
TCTAGTT63050.09.5675177
GGGGTCA31100.09.3189062
TTTCGTA196600.09.3040652
CTTTCGT210750.09.2215471
Produced by FastQC (version 0.11.5)