FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869174
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAT2608.277575E-616.062521
GATCTAG1950.00538861514.2065435
GGTCTGA2000.00637316813.8513795
CTCGGGT2350.001121913813.822121
CGGGGCT2853.2340563E-413.0253561
CTCTAGT2500.001844653912.9396966
CGGGGAT3601.2364562E-512.8896761
CTCGGAT5402.9631337E-912.8896761
ATGGAGT3308.1414015E-512.60366
CTGGGAT2954.3268292E-412.5838181
CGGGAAT4851.3166573E-712.4378741
TCTAGTT2650.002858894212.2061947
GGGAATC4558.712432E-712.1778882
CCGGGAT3208.5743016E-411.6007091
CTCCAAT5203.2562457E-711.6007091
CGGGCAT3601.7643074E-411.6007081
CTAGACT5103.2430762E-610.9183131
CTGGAAT5406.4231226E-610.3117411
CGTCCCA5851.5674796E-610.26966
CGGGCTT4105.876716E-410.1859871
Produced by FastQC (version 0.11.5)