FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13449433
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA41100.012.8299834
GGGGAAT43800.011.9315641
CGGGGAT56300.010.2950941
CGGGAAT71150.09.7489261
GGGAATC55050.09.6650842
AGGTCAT44350.09.5332573
CTGGGAT38400.09.5266081
GAGGTCA49750.09.0714232
CGGGGTT30400.09.0642791
TAATCCC61800.08.9926119
CGGGCAT38550.08.8733371
TCTAGAC54100.08.8677317
GTCATAA64800.08.7973035
CTCGGAT75400.08.758351
CGGGCCT29300.08.5938371
CTAGACT51450.08.5859038
CTGGAAT64050.08.3076411
CCGGGTT25850.08.2704821
GGGGATC39400.08.1989382
CGGGGCT31300.08.044711
Produced by FastQC (version 0.11.5)