FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15241709
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA46600.013.7621154
CTGGGAT44150.011.1915411
AGGTCAT55650.010.4143473
CGGGGAT64850.010.4031681
TCTAGAC72100.010.079647
CCCGGAT44950.09.8297041
CTAGACT71550.09.6260318
TAATCCC69450.09.5067379
CCGGGTT29500.09.5020481
CTGGAAT76150.09.4833311
CGGGAAT81250.09.4728121
GAGGTCA62700.09.3191192
CGGGCAT45750.08.9308861
GGGGAAT46500.08.6846681
CTCGGTT73450.08.6676271
CGGGTAT40450.08.4567051
CGGGGTT29800.08.4498071
GGGGGAT39400.08.3203211
CTGGGGT21750.08.3006391
CATTCTA104000.08.0392714
Produced by FastQC (version 0.11.5)