FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600432
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA47950.013.8703664
CTGGGAT42300.011.2323031
GGGGAAT40050.010.7956321
AGGTCAT51800.010.3632793
CGGGGAT54750.010.2401681
CTGGGGT18550.09.9891811
TAATCCC63800.09.97635659
TCTAGAC69100.09.9673057
CGGGCAT40950.09.5141311
GTCATAA70600.09.4877085
CCGGGAT50750.09.4557191
CGGGAAT74650.09.356141
CTAGACT71000.09.2991728
CTGGGTT21550.09.2600041
GAGGTCA58300.09.2078532
CCGGGGT23850.08.9646491
CGGGGAG57150.08.895631
CGGGTAT37700.08.8219771
GGGGGAT36700.08.8034321
CCGGGTT26350.08.6550551
Produced by FastQC (version 0.11.5)