FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13411955
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49400.016.828784
AGGTCAT53150.014.032593
GAGGTCA58700.013.353262
TAATCCC69200.012.080779
GTCATAA74550.011.5337145
CGGGGAT53800.011.0383671
CGGGAAT71850.010.8441161
TCTAGAC65100.010.1419597
CTGGGAT39400.010.1288511
CCCGGAT34500.010.0526561
CTAGACT64250.09.9824641
CTGGAAT68700.09.7507831
CGGGCAT36800.09.2952651
CGGGCCT24350.09.170141
CCGGGAT44400.09.0952171
CGGGTAT39100.08.9914971
CATTCTA92000.08.9846864
CCCGGTT25950.08.7878171
CGGGGCT31950.08.7732041
GGGGGAT32600.08.7440121
Produced by FastQC (version 0.11.5)