FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13278784
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA41400.013.0807894
GGGGAAT40800.011.062461
CCGGGAT48100.010.766381
CGGGGAT51400.010.7221811
TCTAGAC57950.010.3277487
CTAGACT56300.010.2085848
CGGGAAT68700.010.1659721
CGGGTAT36150.010.1197721
CGGGCAT39000.09.8675361
CGGGGAG56800.09.6191741
TAATCCC60250.09.2239599
GGGGGAT32600.09.181451
GAGGTCA51750.09.1795012
CCCGGGT20800.09.1366061
CCCGGAT32850.09.1115751
ATTCTAG75400.09.0723665
GGGGTAT20500.08.8067971
GGGGATC38050.08.7392232
AGGTCAT50600.08.7309573
CGGGGCT31150.08.6937091
Produced by FastQC (version 0.11.5)