FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15522634
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC219540.14143218219279022TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA59000.018.3575124
AGGTCAT67200.013.4312153
TAATCCC83350.012.8792889
GAGGTCA71050.012.6365552
GTCATAA86250.012.6126385
CGGGGAT58750.012.4536591
GTATGCC101600.011.96837744-45
TATGCCG106700.011.84148846-47
TGCTTGA119300.010.92926358-59
CGGGAAT77400.010.8646681
GCCGTCT114050.010.76599848-49
ATGCCGT115250.010.57147246-47
CCGTCTT121900.010.52080750-51
CGTATGC122800.010.32766144-45
CCGGGAT55400.010.2051961
TAGCTTA123150.010.12474134-35
ACTAGCT125050.010.00889132-33
CGGGTAT46500.09.80849551
TCTAGAC82300.09.7538467
CTAGCTT124800.09.70542632-33
Produced by FastQC (version 0.11.5)