FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrMB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrMB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535828
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA53150.017.607774
AGGTCAT59950.012.837113
GAGGTCA62000.012.56592
TAATCCC77800.012.1495939
CGGGGAT60450.011.4749581
GTCATAA84750.010.8742845
GGGGAAT43850.010.1848081
CCCGGAT41550.09.4907731
CGGGAAT79950.09.3298731
CTGGGGG13900.09.2287641
CCGGGAT57450.09.1796791
TATGCCG92000.09.1126646-47
GGGGGAT40650.08.8827521
CGGGTAT41200.08.8794911
GTATGCC87050.08.75779144-45
CGTACAA154400.08.7379525
CTGGGAT42600.08.5876761
TGCTTGA101050.08.57856858-59
CTAGACT79550.08.478848
CTGGGTT24300.08.4073041
Produced by FastQC (version 0.11.5)