FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088445
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA48150.018.7455484
AGGTCAT54700.013.5480923
TAATCCC64450.013.1922499
GTCATAA71200.012.743665
GAGGTCA60150.012.0836082
CGGGGAT48800.011.9754221
CTAGACT59950.010.4614111
CGGGTAT35600.010.4099861
GGGGAAT36200.09.9749461
CGGGGAG51050.09.9584911
CCGGGAT44650.09.8961671
GGGGATC34400.09.6667492
CGGGAAT62150.09.6324091
ATAATCC95300.09.4699888
CCCAATT68850.09.4541421
CGGGGTT24700.09.4255051
CGGGGGT30050.09.32852651
GGGGTAT20000.09.2648871
CCCGTAT28850.09.222481
GGGGCAT17700.09.1266451
Produced by FastQC (version 0.11.5)