FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12826827
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT139560.10880321376440176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA46000.016.4203974
AGGTCAT53400.012.00982953
CGGGGAT45250.011.655341
GGGGAAT47200.011.0731521
TAATCCC69150.010.8530899
GTCATAA72500.010.6804335
GAGGTCA59850.010.5567892
CCCGGAT42100.010.3830811
CCGGGAT46250.010.27327251
CGGGTAT35450.09.6502111
CGGGAAT64800.09.5321071
GGATAGT69350.09.5233311
CGTACAA152150.09.4604095
TTCGTAC144800.09.3501943
CGGGCAT36400.09.26781751
GGGGATA29050.08.9941652
ATTATAG108550.08.9714645
CTAGACT62800.08.776451
GGGGCAT19000.08.7525581
CTGGAAT62450.08.7495551
Produced by FastQC (version 0.11.5)