FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13159005
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGATCGAACGGGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGG211390.16064284495674255No Hit
GGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGGTCGGCGATGTCCC160480.1219545094784902No Hit
AAGAAACCCAAAAGATCGAACGGGGAGATTCATCGTCAGCGGCGCTGGCT158430.1203966409314382No Hit
CGGGGAGATTCATCGTCAGCGGCGCTGGCTTCGCGTCGGTCGGCGATGTC136750.10392123112651754No Hit
GCAAAACCCAAAGGCGCAATGAAAGTGAAGGTCGGCCTTGGCGCCGACCG133490.10144384016876656No Hit
CTGATACCGTGCGAGTACCGCCGGGCAGCCGGCCGAGCTTCCGGGGGTTT132070.10036473122397932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACC34150.025.1805321
GGTCATA33050.025.1538244
CTCACCA36050.023.5876792
CTAACAT57900.023.3853231
AGGCCCA40900.022.5303548
CATGTGC59000.021.9808655
TAACATG61400.021.1992072
CCCCAGT57350.020.793011
TCACCAG43400.020.140283
CACCAGG45350.019.4837744
ACATGTG68750.019.0710164
CCGTCAT44100.018.3125063
CCCCCAT55050.018.2959751
GAGGTCA39050.018.004382
ATGTGCG79750.017.9293696
GTCGGAT76250.017.5705871
CCCAGTC71150.017.1591452
AACATGT78700.016.8409613
GGGTCCT33550.016.7080942
CATTGAC49800.016.310097
Produced by FastQC (version 0.11.5)