FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13661720
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA50800.016.9259554
TAATCCC69250.011.5233449
CGGGGAT52700.011.2692891
AGGTCAT59300.011.05513
GTCATAA77850.011.0447695
GGGGGAT31800.010.6078751
GAGGTCA66200.010.2616292
GGGGAAT41450.010.08684351
CTAGACT64900.09.8097341
CGGGGAG57100.09.568831
GGGGATC39900.09.524792
GGATAGT63600.09.2632141
CGGGTAT39700.09.0953661
CGGGAAT72600.08.9001921
CTGGAAT67850.08.7530071
GACCCTA30050.08.6937517
CCCAATT75350.08.449261
TCTAGAC66900.08.3780887
GGGAATC55050.08.3705252
CGGGCAT44400.08.3465841
Produced by FastQC (version 0.11.5)