FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13873810
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT141390.10191144321567039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA63250.022.532694
TAATCCC88500.016.369859
GTCATAA92200.015.818075
AGGTCAT73900.015.7497613
GAGGTCA77550.015.1309952
ATAATCC127650.011.8329898
CGGGGAT50350.011.1348651
GGGTCAT27100.010.5180273
GGATAGT74850.010.4735661
GGGGTCA28400.010.371122
CGAGGTC115900.010.2894621
GGGGAAT42050.010.1690161
CGTACAA167500.010.0116565
TCATAAT149300.09.768186
CCGGGAT50400.09.7097741
TTCGTAC164400.09.7093423
CGGGAAT68050.09.7048561
GGGGGAT33300.09.702131
CCCAATT100550.09.5449081
GTTTAAT161400.09.5377051
Produced by FastQC (version 0.11.5)