FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12312791
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT129420.10511020612629582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49350.016.845774
TAATCCC65700.013.0858549
GTCATAA69800.012.318655
AGGTCAT57300.011.9384483
GAGGTCA61800.011.6072262
ACTCTAG26450.011.1354055
CGTACAA144050.010.4870625
TTCGTAC136650.010.3944513
CGGGTCT40950.010.3255491
CGGGGAT48350.010.317391
GGGGATC33200.09.5868662
TCGTACA158200.09.1887184
ATAATCC96750.09.1316828
GTTTAAT121500.09.1108031
TTTCGTA161650.09.0807722
TACAAAT188250.08.8817087
GGGGGAT29100.08.8161271
CTTTCGT181150.08.7596181
CGGGCAT38050.08.6153211
CACCTTA38400.08.5360685
Produced by FastQC (version 0.11.5)