FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11627224
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA49050.017.1426854
TAATCCC67200.012.9351189
AGGTCAT54500.012.8134313
GAGGTCA56900.012.6069282
GTCATAA70200.012.2485375
CGGGGAT46000.010.01861
GGGGGAT27250.09.9380581
CGGGAAT60350.09.6045211
GGGGCTA9902.1827873E-109.5970872
CTAGACT63500.09.4273581
CGGGTCT40350.09.4197561
ATAATCC95000.09.3498928
CGGGGAG46300.09.2353781
CGGGTAT32850.08.9670481
GGATAGT57050.08.9108941
GACCCTA33050.08.9106387
CCCGGAT38950.08.9044791
CCGGGAT46500.08.8891341
CGGGGCT25700.08.6887631
GTTTAAT127500.08.5705921
Produced by FastQC (version 0.11.5)