FastQCFastQC Report
Sat 28 Jul 2018
H5CCNBCX2_l01_n01_AntOrAL1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCNBCX2_l01_n01_AntOrAL1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823180
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC321200.23236332016222028TruSeq Adapter, Index 1 (100% over 50bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT178880.12940582413019291No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA175480.12694618749086678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG87600.026.6269575
CTCTAGT94450.024.5947046
TCTAGTT108350.021.3056587
GGTCATA60300.020.6409514
TATGCCG109750.020.449646-47
CTAGTTG114900.019.5950288
GTACTCT119050.019.1938193
CACCTTA97800.019.0896435
CGTATGC119350.018.98381644-45
TACTCTA125950.018.5949254
TGTACTC127200.018.0013732
CCGTCTT128200.017.58068350-51
CCTTAGA107650.017.5171747
AGTCACA129650.017.47565328-29
CCAGTCA129100.017.45812226-27
GTATGCC104250.017.31390844-45
GAGCACA211500.017.2929049
TGCTTGA130700.017.20806358-59
ACTACCC52450.017.027356
TAGTTGT138550.016.5587739
Produced by FastQC (version 0.11.5)