FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7H8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7H8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCCGTTGGCGTTGTCCTCGGTTACCTGATGGGTCTGGAAGAACTGTG14.0No Hit
GGCAAGAGCCAGGCCTCGATGAGTAGGACTGATCGTCGGTAGCAGCAAAA14.0No Hit
CTTCCGATCTGACTGGGGAGACGTGTGCTCTTCCGATATGAATTGTGAGA14.0No Hit
GTGTGCATTCAACTCACTGAGTTGAATATTTCCTTTGATTGAGTAGTTTT14.0No Hit
GATGAAAAGGCTTTTTAGTGCCGTCATGACAATAGTAGCTATTACCACAC14.0No Hit
TGCATGGCTTCGTTGGTGCCCTCTACCTCGATCACCGCGGCGATCTTATT14.0No Hit
GAATTAATCTATAAAAATACAAACTACTGATAAGATCCTTTCTATGTTTA14.0No Hit
GAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCG14.0No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC14.0No Hit
TTTAAGTAGTTTTTCTAATGTCATTCAACTATAAAGAGGTAGAGTTCACT14.0No Hit
TTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATG14.0No Hit
GCTATTATATTCCGGCGTCGAGCCTGCTTTGTACGCTCTAATTTTTTGAA14.0No Hit
GGAGACGTGTGCTCTTACGATCTCCCGTGGAGAAGTGTGCTCTTACGAAT14.0Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCAATATGACAATCGTGCAGGTCGGAACTTAAAAGACAAGGAATTTCGAT14.0No Hit
TTCTCATCCTACCCACCAGTGTCGGTTTACGGTACGGTCTGCGTAAGCTG14.0No Hit
GTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGATTAATGAAAAC14.0No Hit
GAGTGTTTTTGATGCTTCTGGGTTGGGTGAGTGTGTGAACCGATCATGTA14.0No Hit
CCAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAGGTTAGTTTT14.0No Hit
CTGACTATCAAATACGAATGCCCCCGACTTTCAATGAAAAAAATTACTCC14.0No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA14.0No Hit
CAATCAATTCGCGAAAGACGCGGAATCATCCCCGGATCAACATGCGGAAT14.0No Hit
GGAAGGGACGCATTTATTAGATAAAAGGTCGTTGTTCTGATCATTCATGA14.0No Hit
ATTTTTGTATAGGTATACCTATAGGATAAAAAGGTAAAATTTTGAGTAAG14.0No Hit
GAATTAACCAGACAAACCGCTCCACCAACTAAGAACCGCCATGCAAAACC14.0No Hit
GTTCCTGGGGAAGGGTCGTCCGCCCAGGGAAAGTCGGGGCCTAAGGTGTA14.0No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)