FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7H6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7H6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGTACCATAAGCCGTGTTAGTCCCAGTTTGATGTGAACAATGATG15.88235294117647No Hit
TATTTGCTCTGCTTGTAAGGCCAAAGCCAGACCACAAATATCTAACGTAC15.88235294117647No Hit
GAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTTCCTAACTGC15.88235294117647No Hit
CAACAGACTTGATCTCAGTGGTCAATCCTGTGGGAGCAAAGGTCACACCC15.88235294117647No Hit
GTGTTGATGCTGATCTTAGATACAGTTATCTGATGAACACTAGCATCAGT15.88235294117647No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCATCCTTTGAGCACTC15.88235294117647No Hit
GCAATACGATGGTCCCTGCGGATGCTAACGCAATGAGATTTCTGCCCAGT15.88235294117647No Hit
CACTTTTTACATCACCAACACGCTTTTCAAGTTGGAAACTGGATGCGGAC15.88235294117647No Hit
CCCTGGGAAGATCGGAAGAGCACACGTCTCCCCCGGAAGATCGGAAGAGC15.88235294117647Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCTGGGCATGCCGAAGACGATGCAACTGGCTTCGTCTTGTACATAGTT15.88235294117647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15.88235294117647No Hit
GTTTAGTGGACTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTCGCC15.88235294117647No Hit
TATTTAAGATGCTCATTTATGTCTCTGCATGTAGCTAGGTTTAGCCGGGT15.88235294117647No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCAAGCTCACACTCGAACCCTTCA15.88235294117647No Hit
GTAGTAGCAAATATTCAAATGAGAACTTTGAAGTCCGAAGAGGGGAAAGG15.88235294117647No Hit
CGCTAATCATTGGCTGTACCCGATAGAACTCGCGTCCGAGCTCCAGCTAT15.88235294117647No Hit
GGGGAAAGCCATACCCGAAATCACGATCCTGTCGTGGTGTGCCGGCTCCC15.88235294117647No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)