FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7H2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7H2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAAACATCGTGTAATCGAGGAAGGTGTGTAATCTTTGTTTTTTAGG14.0No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA14.0No Hit
TCCTCTTCGTTTGCATCAGGAAAGAGCGAGAATGGCGGGAGGAGGAAACT14.0No Hit
TCCAAGAATTTTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTC14.0No Hit
AGGAGACAGTCCTGAGTGCTTACTAGCAGATACCGGATCTGGAAAAGTGA14.0No Hit
CCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTC14.0No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC14.0No Hit
AAACTGTGGTTAAGGACTTAATCTTTCTTAACGTGAAAGTGTTGGTAGTT14.0No Hit
AATGAGCTATGACCGCCTCCGAGTGGTATTCTTGGAAGAAACACTGCAGA14.0No Hit
GGCGGGGGAGTTAACAAACCGGTCTTGAAAGTCCGGCCATAGAAGGTGAT14.0No Hit
CAATCTCTTGTCTTTCGTCTCCAATGTTCTGCTTCTTCTCGTTGCGATCT14.0No Hit
TCCCTAAAGAGATCGGAAGAGCACACGTCTCCCTAAAGAGATCGGAAGAG14.0Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTAGTGGAGAGATAGTTTATGCTCTCTGAGATTCCTGAGGACAAAGTCTG14.0No Hit
CGTGGGGGCAGCGGCTGCCCTGGGGGCGTGGCTATGTGGTGCACGTGCAG14.0No Hit
CCGAGAAAATGTAATGGATCAAGTTCACCCCCGCATGTCGGTACGCTCCC14.0No Hit
ATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAAT14.0No Hit
GTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTT14.0No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG14.0No Hit
GGGTAGGACGTGTCGGCTGCTTTGTTGTGGCGTCACACGGAATCGAGAGC14.0No Hit
CTTTTAAAGCTCTTAGCTTAGTCTCGATTATCGCTTGTCCGCAGAACTGG14.0No Hit
ACCATACTCCCCACGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14.0No Hit
GTTTAGGGAGACGTGTGCTCTTCCGATCTGTTTAGGGAGACCTGTTCTCG14.0Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCGGGCTCTGCCACGGTGTTAAGAAGAGGAATTGAACCTCTGACTGT14.0No Hit
GCCCCGGGAGACGTGTGCTCTTCCGAACTGCTCAGGGAGAAATCGCCGAG14.0Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCC14.0No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)