FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGGGCGTCGGCCTGTGGGCTCCCCATTAGACCCGTCTTGAAACACGG14.761904761904762No Hit
CCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCATAACCGCATCAG14.761904761904762No Hit
CTTTACAGGGGGCCGGGTCGTGCCAGCGGCCGGGGTTGATTAAAGAATAA14.761904761904762No Hit
AGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCAT14.761904761904762No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT14.761904761904762No Hit
GGCGAGAGCCGTACACAGGGAACCTTACCACTCTTCGTACATGAAAGGTC14.761904761904762No Hit
CCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGC14.761904761904762No Hit
CTTCCGATCTCCACAGGGAGACGTGTGCTCTTCCGATATGCACCGGGATA14.761904761904762Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGAT14.761904761904762No Hit
CTCAGGTTCTCTATAGCACTCCCGTCATAGGGTTCTTTTCACCTTTCCCT14.761904761904762No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT14.761904761904762No Hit
GCTGAGTTTTAATGCTGCATTAGACTTCTCAAACTTTTGGTCAAAAGACT14.761904761904762No Hit
GGGGCCAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGCGGGTCGATCC14.761904761904762No Hit
GCTAATGGAGATTCTAAGGTTTTTTACCTTAAGATGAAGGGAGATTATCA14.761904761904762No Hit
ACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCT14.761904761904762No Hit
CGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAG14.761904761904762No Hit
GTCTACGAGTCGGGTTGTTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT14.761904761904762No Hit
CTCCAGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCTTGAGACACGG14.761904761904762No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG14.761904761904762No Hit
GGATAACATCATAGGATTTCGATCCTATGGTGTTGGCCTTCGGGATCGGA14.761904761904762No Hit
TGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGA14.761904761904762No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)