FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTGCTCTTCCGATCTCGTCGGGGGGAGACGTGTGCTCTTCCGCTCTC12.5Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12.5No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT12.5No Hit
TGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTT12.5No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12.5No Hit
GGATACCATCAGAAGAACCATCCAAAAATACATACCTAATTTTTGTTATA12.5No Hit
GTTGTAAAGCATGCTGGAGGTATCACAAGAGCGAATGCTGACGTGAGTAA12.5No Hit
CCATAATAGATCGGAAGAGCACACGTCTCCCATAATAGATCGGAAGAGCA12.5Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12.5No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC12.5No Hit
GCCTATTACCGCGCGCTGGAGCTCGGCGCCCAACCCATCGACATCCCCAC12.5No Hit
GTATAAAGTTGCAACTTGCAAGCCTTATTGTTTTGCCAGCCTAATATTTC12.5No Hit
TCTTCACGGAGAATTCAATTTCGCTGAGCATATCCTGGAGACAGTGGGGA12.5No Hit
TCTGTATACTGTATGGTTGACTACGATCGTTCCTAGCATAGACTTCAGTG12.5No Hit
CATCAATAGAGATTCCCCTAGTAGCGGCGAGCGAACGGGGAGGAGCCGAC12.5No Hit
GCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAG12.5No Hit
TACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGA12.5No Hit
CACGTCTCCCGACCAGATCGGAAGAGCACACGTCTCCCGACCAGATCGGA12.5Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAA12.5No Hit
TCCCTATGGAGATCGGAAGAGCACACGTCTCCCTATGGAGATCGGAAGAG12.5Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTGACAGGGGGCCGGGTCGTGGGGGCGGGGCGGGTGGTTTT12.5No Hit
CTGATACGGGAGACGTGTGCTCTTCCGATCTTCCTGGGGAGACGTGTGCT12.5Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGT12.5No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG12.5No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT12.5No Hit
CAGTTGCAGCGGCCTGGTATCTGCGACTGCCAATAGCTTTGTAGTGCGTG12.5No Hit
ACCCAGAAGTGCCGAGCATGGGAGACACACGGCGGGTGCTAACGTCCGTC12.5No Hit
CTGTTACGTCACTTTCAACCTGCTCATGGATAGATCACTCGGTTTCGGGT12.5No Hit
ACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAAC12.5No Hit
TCTCTAGGGTGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG12.5No Hit
GATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCG12.5No Hit
CCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGG12.5No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG12.5No Hit
GGCAAGAGCCCCCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAAC12.5No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG12.5No Hit
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTTACCGGGGCTTCGATCA12.5No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG12.5No Hit
ATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTA12.5No Hit
AGGCTGGGTCCTTGAAGTGAAGCCCAGGGCCTGCCCAAAGTAGGGTATCT12.5No Hit
ATAAAAGACTTATTAAAAGAGTCGATTCAGGGGCATCCATCTCAGTTTGG12.5No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers