FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACAAATGCTCTAAGCTAGGAAAGGTTTTCCACATCCACAGTCAACGA12.380952380952381No Hit
ATCAAAAGAAAGGTTCAACTTTGTTAGCTGAGTAGATACAGCATAAACAA12.380952380952381No Hit
AAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCG12.380952380952381No Hit
GGAGCGGGCAATGCCAGCGCAGTAGGACCCTTGGTGGAGCGCACCAGCCG12.380952380952381No Hit
GACTTAAATGGTAATTTTATAAAAGAGTACCCTTGCGCCCGAGAGGCCGG12.380952380952381No Hit
GAATATAGACAGGAGTAAAGAGGAATCTTTTGAAAGTGATACCAGGAGTT12.380952380952381No Hit
GTCCTGGAGAACAAGCAGCAGGGTCAGCAAGCGAAGCAAAGGTTGCTGGT12.380952380952381No Hit
ATATGACAACGACATTAAGCTTTTGACCCTTCTTACAGTTATCTTCATTG12.380952380952381No Hit
TTGCTAGACACAGTTAAAGAAAGCACAGAGATATTGATAAGATGACATTT12.380952380952381No Hit
CTCCAGTACCTGTTACGGCACCTTCAACCTGGCCATGGATAGATCACTCG12.380952380952381No Hit
GTGGAAGACAGTGTGTTGATTCCTCAAGGATCTAGAACTAGAAATACCAT12.380952380952381No Hit
AGAAAACTCAACACCACCACCCCCCCTACCACTCAGCACACACAAAAGTT12.380952380952381No Hit
TCTTAGAATTAAGTTGAGAAAGAAATATCCTAGCAAAATCCGAGAAAATT12.380952380952381No Hit
CTTGCACATTAAGAAATTGAGATCTGACATATCAGTGAACTGCTTGTTCG12.380952380952381No Hit
ATGATTGCGCCACTGCACTCCAGCCTGGGTAACAAAAGGAGACCCCGTCT12.380952380952381No Hit
CGTCTCCCGTCCCAGATCGGAAGAGCACACGTCTCCCGTCCCAGATCGGA12.380952380952381Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAAGTGCATCAGGTGGATGCCTTGGCGATGATAGGCGACGAAGGACGTGT12.380952380952381No Hit
CACCGTGACGGACTTGGCCAGATTCCTTGGCTGGTCAACGTCATGACCCT12.380952380952381No Hit
TAAATATTTTGGCCTGGTTGACAGACCTGGCAGAATTAGGACTACAATGC12.380952380952381No Hit
GCATAGGGAGACGTGTGCTCTTCCGATCTGCATAGGGAGACATGTGCTCT12.380952380952381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTAAGAAGAGGAATTGAAAAGTTTTATGCGATTACCGGGCTCCCTACCT12.380952380952381No Hit
CTCTGCCATGGTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT12.380952380952381No Hit
GCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA12.380952380952381No Hit
ATCTGCCACAGAACATAGGATAAAAAGGTATTAAATCAGCTTCCTCCATT12.380952380952381No Hit
AGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTT12.380952380952381No Hit
CCTCTACCGTTTGCTCCCATTTAGCTTTTAGACAATTAGAAGTTGTCATT12.380952380952381No Hit
AGACTGGATTAAGAAAATGTGGCACATATACACCATGGAATACTATGCAG12.380952380952381No Hit
ATGAATTAATGAGTGAATGAATGAATGAGTGAATGAGTGAATGAATGAGT12.380952380952381No Hit
GAGAAAGAGAGCTGATGCAGACAAGAACGACAAGTCTGTGAAGGACCTTG12.380952380952381No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTT12.380952380952381No Hit
ATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCC12.380952380952381No Hit
ATTTTCTACTTCTGCAATGTTCGTGGGTAGAAGTATATTTTTTGTGTGTT12.380952380952381No Hit
AGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGCT12.380952380952381No Hit
CTTCCGATCTGTGTAGGGCATTAAAAATGCTGATGATGTTTGCTCATCTA12.380952380952381No Hit
CTGCTGGTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGT12.380952380952381No Hit
CTCAGCAGGGAGACGTGTGCTCTTCCGATCTAATCAGGGAGACGTGTGCT12.380952380952381Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCATCAGATGGTTTGTCATAATCATCGTAAATCTCATCCTTTGAGAATGC12.380952380952381No Hit
GGTTCTTTGACAACGCTATCGGTGTTAATGTACCTCGCTCACGGTTCTTG12.380952380952381No Hit
CAATATGGAGGAGTGGATGAATATGATGATGATGATGGATTTTGGGAATG12.380952380952381No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTAATAGTCAGA12.380952380952381No Hit
CGTCACTCTATACTTGGGGCAAGTCCAGGGGCTATTAGGGTGGTCACCTT12.380952380952381No Hit
GCCATAGCCAGTTGCTGTTTGTTTATGACACAATGCTTTACTTCGCCGTC12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers