FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGATCGGAAGAGCACACGTCTCCCTGACCAGATCGGAAGAGCACACGTCT15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15.263157894736842No Hit
GGTAGTTATTGTCACTACCTCCCGGTGTCAGGATTGGGTAATTTGTGCGC15.263157894736842No Hit
AGCTTTGCGCTGGCGGCGCTCATCGGCGCGGTCTCGGGCCTGCTCATCGC15.263157894736842No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG15.263157894736842No Hit
GTGTTTGGTCCGAAGTCTCACTGGCAGAGAAATTGAATACCGGACGGACT15.263157894736842No Hit
GTGCTCTTCCGATCTCATCCGGGAGACGTGTGCTCTTCCGATCTCATCCG15.263157894736842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGGTCG15.263157894736842No Hit
AAGTAGTACAGCGGAAGTCGCTCAGGCCCGCATAGCCGTCACCGGCGAAC15.263157894736842No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15.263157894736842No Hit
TGCATAAAGTAGGCGCTCGCAGTCCCCACAAATGGGACACCAATGTTGTA15.263157894736842No Hit
GCACAGGGAGACGTGTGCTCTTCCGATCTGCACAGGGAGACGTGTGGGGA15.263157894736842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCTGAACAGGGGGCCTACGGGTCCTGAACTTCTTTTCCCAGAG15.263157894736842No Hit
CACGTCTCCCACGACAGATCGGAAGCGCACAAGTCTCAAAAGACAGATGG15.263157894736842No Hit
GGCGCTGGCGACGATGAGCTTGGCGCGCATGAGATATCCTTTGAAGTATG15.263157894736842No Hit
CTCCCTTACTTTCTAATTTATTTTTTAACAACCGACACTTTCATTAATTT15.263157894736842No Hit
TGAGGAAGCCGAAGAAGACTTTGAAAAAGCCTTGAACATACTCGACATCA15.263157894736842No Hit
TGCCCCGACGGCCGGGTATAGGTCGCGCGCGTAAGCGCCCTCCATTTTCG15.263157894736842No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)